William Querido (wquerido)

William Querido

Assistant Professor

Charles V. Schaefer, Jr. School of Engineering and Science

Department of Biomedical Engineering

Edwin A. Stevens Hall 208

Education

  • Ph.D. (2014) Federal University of Rio de Janeiro (Biophysics)
  • Ph.D. (2014) University of Haute Alsace (Cell Biology)
  • M.S. (2011) Federal University of Rio de Janeiro (Biophysics)
  • B.S. (2009) Federal University of the State of Rio de Janeiro (Biomedicine)

Research

- Bone and other mineralized tissues in health and disease
- Infrared spectroscopy and hyperspectral chemical imaging of biological tissues
- Relationships among bone composition, structure, and mechanical function
- Machine learning and multivariate analysis of high-dimensional spectroscopic data
- Development of spectroscopic approaches for assessing tissue quality and biomaterial interfaces

General Information

Tissue biomineralization produces complex composite materials in which the organization and composition of organic molecules and inorganic minerals determine tissue structure and function. Dr. Querido’s research focuses on understanding bone and other mineralized tissues using infrared spectroscopy, hyperspectral chemical imaging, and complementary structural and mechanical approaches. His laboratory investigates how compositional and structural properties across multiple length scales contribute to bone tissue quality, mechanical function, and fragility. The group also develops high-resolution and non-destructive spectroscopic approaches, together with multivariate analysis and machine learning, to extract biologically and mechanically meaningful information from complex spectral data. This work seeks to advance our understanding of mineralized tissue biology and enable improved approaches for assessing tissue quality and designing biomaterials and regenerative strategies.

Experience

Department of Biomedical Engineering, Stevens Institute of Technology
2024 – Present: Assistant Professor, Tenure-Track

Department of Bioengineering, Temple University
2021 – 2024: Assistant Professor, Research-Track
2020 – 2021: Adjunct Assistant Professor
2017 – 2021: Postdoctoral Fellow

Institute of Biomedical Sciences, Federal University of Rio de Janeiro
2015 – 2016: Postdoctoral Fellow

Honors and Awards

Dr. Gerald Finerman Sports Medicine Award
MTF Biologics, 2026

Recognition for Outstanding Teaching Evaluations
Stevens Institute of Technology, Office of the Senior Vice Provost for Academic Innovation and Faculty Affairs; Spring 2025, Fall 2025

Grants, Contracts and Funds

SES Seed Funding Programs, ACE Program
“ACE: Development and Validation of Biomimetic Bone Phantoms for Spectroscopy Standardization”
Sep 2026 – Aug 2027, Role: PI

Semcer CHI AIMS Seed Funding
“Neuromuscular and Behavioral Biomarkers of Mobility Decline”
“Endothelial Biomarkers of Early Metabolic Dysfunction”
“Vascular–Cognitive Biomarkers of Early Neurodegeneration”
Sep 2026 – Aug 2027, Role: Co-I

SES Seed Funding Programs, SPRINT Program
“SPRINT: Bone-Inspired Design Principles for Damage-Tolerant Cementitious Materials”
Mar 2026 – Feb 2027, Role: Co-PI

MTF Biologics, Innovation in Allograft Translational Research Grant, Established Investigator Grant
“Non-destructive Quality Assessment of Tendon Allografts Using NIR Fiber Optic Spectroscopy”
Jan 2026 – Jan 2029, Role: PI

Pennsylvania Department of Health, CURE Program
“Sex-Specific Differences in Bone Composition and Relationship to Bone Strength”
May 2023 – Dec 2026, Role: PI

NIH/NIAMS, R21
“Multifactorial Contribution of Bone Nanoscale Composition to Tissue Quality in Osteoporosis”
Jan 2023 – Nov 2025, Role: PI

Patents and Inventions

Arthroscopic Probe Device, System and Method
U.S. Patent No. 12,629,013, May 19, 2026, Role: Co-Inventor

Selected Publications

Journal Article

  1. Ibrahim, R.; Petrocelli, N.; Ratnamani, M. P.; Lapinski, M.; Sarhaadei, E.; Querido, W.; Wang, H. (2026). A Channeled Chitosan-Based Polyelectrolyte Scaffold Platform for Localized Osteogenic Cue Delivery and Spatial Control of Mineralization in Centimeter-Scale Constructs. ACS Applied Bio Materials. American Chemical Society (ACS).
    https://doi.org/10.1021/acsabm.6c00665.
  2. Di Pede, R.; Spurri, A.; Kust, S. J.; Block, J.; Querido, W.; Santana, R.; Semeniuk, D.; Pleshko, N. (2026). Infrared Spectroscopy for Determining Bone Graft Healing and Readiness in Tissue Models.. The International journal of oral & maxillofacial implants (0 ed., vol. 0, pp. 1-27).
    https://doi.org/10.11607/jomi.11616.
  3. Rondon, A. J.; Querido, W.; Tzeuton, S.; Singh, A.; Patel, N.; Karchner, J.; Fertala, A.; Pleshko, N.; Abboud, J. A. (2026). Skin keratin correlates with bone and tendon collagen quality: a proof-of-concept clinical study using FTIR spectroscopy in shoulder arthroplasty patients.. European journal of orthopaedic surgery & traumatology : orthopedie traumatologie (1 ed., vol. 36).
    https://doi.org/10.1007/s00590-026-04881-0.
  4. Spurri, A.; Arefin, M. S.; Querido, W.; Kust, S. J.; Santos, M.; Schaer, T. P.; Fentaw, B.; Proca, D.; Barnes, L.; Patil, C.; Pleshko, N. (2026). Optimization of Data Collection for Visible-Near-Infrared Fiber Optic Spectroscopy of Osteochondral Tissues in Hydrated Environments.. Applied spectroscopy (5 ed., vol. 80, pp. 433-445).
    https://doi.org/10.1177/00037028251411328.
  5. HassanMazandarani, A.; Masterson, J. M.; Querido, W.; Steplewski, A.; Zhang, Y.; Huynh, C.; Garcia, M. M.; Fertala, A.; Pleshko, N. (2026). Optical photothermal infrared spectroscopic assessment of microplastics in tissue models and non-digested human tissue sections.. The Analyst (6 ed., vol. 151, pp. 1774-1785).
    https://doi.org/10.1039/d5an00241a.
  6. Montoya, C.; Babariya, M.; Ogwo, C.; Querido, W.; Patel, J. S.; Melo, M. A.; Orrego, S. (2025). Synergistic effects of bacteria, enzymes, and cyclic mechanical stresses on the bond strength of composite restorations. Biomaterials Advances (vol. 166, pp. 214049). Elsevier BV.
    https://doi.org/10.1016/j.bioadv.2024.214049.
  7. Querido, W.; Shanas, N.; Radway, A. P.; Jones, B. C.; Ispiryan, M.; Zhao, H.; Hast, M. W.; Rajapakse, C. S.; Pleshko, N. (2025). The Multifactorial Relationship Between Bone Tissue Water and Stiffness at the Proximal Femur.. Calcified tissue international (1 ed., vol. 116, pp. 33).
    https://link.springer.com/article/10.1007/s00223-024-01327-9.
  8. Dev, I.; Mehmood, S.; Pleshko, N.; Obeid, I.; Querido, W. (2024). Assessment of submicron bone tissue composition in plastic-embedded samples using optical photothermal infrared (O-PTIR) spectral imaging and machine learning. Journal of Structural Biology: X (pp. 100111).
    https://www.sciencedirect.com/science/article/pii/S2590152424000163.
  9. Reiner, E.; Weston, F.; Pleshko, N.; Querido, W. (2023). Application of Optical Photothermal Infrared (O-PTIR) Spectroscopy for Assessment of Bone Composition at the Submicron Scale.. Applied spectroscopy (11 ed., vol. 77, pp. 1311-1324).
    https://journals.sagepub.com/doi/10.1177/00037028231201427.
  10. Ohnishi, T.; Tran, V.; Sao, K.; Ramteke, P.; Querido, W.; Barve, R. A.; van de Wetering, K.; Risbud, M. V. (2023). Loss of function mutation in Ank causes aberrant mineralization and acquisition of osteoblast-like-phenotype by the cells of the intervertebral disc.. Cell death & disease (7 ed., vol. 14, pp. 447).
    https://www.nature.com/articles/s41419-023-05893-y.
  11. Falcon, J. M.; Kandel, S.; Querido, W.; Morman, J.; Patel, J.; Miller, L. M.; Mauck, R. L.; Pleshko, N. (2022). Near infrared spectroscopic assessment of engineered cartilage for implantation in a pre-clinical model. Journal of Cartilage & Joint Preservation (1 ed., vol. 2, pp. 100038).
    https://doi.org/10.1016/j.jcjp.2022.100038.
  12. Querido, W.; Zouaghi, S.; Padalkar, M.; Morman, J.; Falcon, J.; Kandel, S.; Pleshko, N. (2022). Nondestructive assessment of tissue engineered cartilage based on biochemical markers in cell culture media: application of attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy. The Analyst (8 ed., vol. 147, pp. 1730-1741). Royal Society of Chemistry (RSC).
    https://pubs.rsc.org/en/content/articlelanding/2022/an/d1an02351a.
  13. Kandel, S.; Querido, W.; Falcon, J. M.; Zlotnick, H. M.; Locke, R. C.; Stoeckl, B.; Patel, J. M.; Patil, C. A.; Mauck, R. L.; Pleshko, N. (2022). In Situ Assessment of Porcine Osteochondral Repair Tissue in the Visible-Near Infrared Spectral Region.. Frontiers in bioengineering and biotechnology (vol. 10, pp. 885369).
    https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2022.885369/full.
  14. Kim, M.; Koyama, E.; Saunders, C. M.; Querido, W.; Pleshko, N.; Pacifici, M. (2022). Synovial joint cavitation initiates with microcavities in interzone and is coupled to skeletal flexion and elongation in developing mouse embryo limbs.. Biology open (6 ed., vol. 11).
    https://pubmed.ncbi.nlm.nih.gov/35608281/.
  15. Shanas, N.; Querido, W.; Oswald, J.; Jepsen, K.; Carter, E.; Raggio, C.; Pleshko, N. (2022). Infrared Spectroscopy-Determined Bone Compositional Changes Associated with Anti-Resorptive Treatment of the oim/oim Mouse Model of Osteogenesis Imperfecta.. Applied spectroscopy (4 ed., vol. 76, pp. 416-427).
    https://pubmed.ncbi.nlm.nih.gov/34643134/.
  16. Falcon, J. M.; Chirman, D.; Veneziale, A.; Morman, J.; Bolten, K.; Kandel, S.; Querido, W.; Freeman, T.; Pleshko, N. (2021). DMOG Negatively Impacts Tissue Engineered Cartilage Development.. Cartilage (2_suppl ed., vol. 13, pp. 722S-733S).
    https://pubmed.ncbi.nlm.nih.gov/33100027/.
  17. Afara, I. O.; Shaikh, R.; Nippolainen, E.; Querido, W.; Torniainen, J.; Sarin, J. K.; Kandel, S.; Pleshko, N.; Töyräs, J. (2021). Characterization of connective tissues using near-infrared spectroscopy and imaging. Nature Protocols (2 ed., vol. 16, pp. 1297-1329). Springer Science and Business Media LLC.
    https://doi.org/10.1038/s41596-020-00468-z.
  18. Querido, W.; Kandel, S.; Pleshko, N. (2021). Applications of Vibrational Spectroscopy for Analysis of Connective Tissues. Molecules (4 ed., vol. 26, pp. 922). MDPI AG.
    https://doi.org/10.3390/molecules26040922.
  19. Querido, W.; Shanas, N.; Bookbinder, S.; Oliveira-Nunes, M. C.; Krynska, B.; Pleshko, N. (2020). Fourier transform infrared spectroscopy of developing bone mineral: from amorphous precursor to mature crystal. The Analyst (3 ed., vol. 145, pp. 764-776). Royal Society of Chemistry (RSC).
    https://pubs.rsc.org/en/content/articlelanding/2020/an/c9an01588d.
  20. Ailavajhala, R.; Querido, W.; Rajapakse, C. S.; Pleshko, N. (2020). Near infrared spectroscopic assessment of loosely and tightly bound cortical bone water. The Analyst (10 ed., vol. 145, pp. 3713-3724). Royal Society of Chemistry (RSC).
    https://pubs.rsc.org/en/content/articlelanding/2020/an/c9an02491c.
  21. Kandel, S.; Querido, W.; Falcon, J. M.; Reiners, D. J.; Pleshko, N. (2020). Approaches for In Situ Monitoring of Matrix Development in Hydrogel-Based Engineered Cartilage.. Tissue engineering. Part C, Methods (4 ed., vol. 26, pp. 225-238).
    https://www.liebertpub.com/doi/abs/10.1089/ten.tec.2020.0014?journalCode=tec.
  22. Shanas, N.; Querido, W.; Dumont, A.; Yonko, E.; Carter, E.; Ok, J.; Karchner, J. P.; Barbe, M. F.; Ali, S.; Patil, C.; Raggio, C.; Pleshko, N. (2020). Clinical application of near infrared fiber optic spectroscopy for noninvasive bone assessment.. Journal of biophotonics (4 ed., vol. 13, pp. e201960172).
    https://pubmed.ncbi.nlm.nih.gov/31957205/.
  23. Karchner, J. P.; Querido, W.; Kandel, S.; Pleshko, N. (2019). Spatial correlation of native and engineered cartilage components at micron resolution.. Annals of the New York Academy of Sciences (1 ed., vol. 1442, pp. 104-117).
    https://pubmed.ncbi.nlm.nih.gov/30058180/.
  24. Querido, W.; Ailavajhala, R.; Padalkar, M.; Pleshko, N. (2018). Validated Approaches for Quantification of Bone Mineral Crystallinity Using Transmission Fourier Transform Infrared (FT-IR), Attenuated Total Reflection (ATR) FT-IR, and Raman Spectroscopy. Applied Spectroscopy (11 ed., vol. 72, pp. 1581-1593). SAGE Publications.
    https://doi.org/10.1177/0003702818789165.
  25. Wischmann, J.; Lenze, F.; Thiel, A.; Bookbinder, S.; Querido, W.; Schmidt, O.; Burgkart, R.; von Eisenhart-Rothe, R.; Richter GHS; Pleshko, N.; Mayer-Kuckuk, P. (2018). Matrix mineralization controls gene expression in osteoblastic cells.. Experimental cell research (1 ed., vol. 372, pp. 25-34).
    https://pubmed.ncbi.nlm.nih.gov/30193837/.
  26. Jongwattanapisan, P.; Terajima, M.; Miguez, P. A.; Querido, W.; Nagaoka, H.; Sumida, N.; Gurysh, E. G.; Ainslie, K. M.; Pleshko, N.; Perera, L.; Yamauchi, M. (2018). Identification of the effector domain of biglycan that facilitates BMP-2 osteogenic function.. Scientific reports (1 ed., vol. 8, pp. 7022).
    https://www.nature.com/articles/s41598-018-25279-x.
  27. Querido, W.; Falcon, J. M.; Kandel, S.; Pleshko, N. (2017). Vibrational spectroscopy and imaging: applications for tissue engineering. The Analyst (21 ed., vol. 142, pp. 4005-4017). Royal Society of Chemistry (RSC).
    https://pmc.ncbi.nlm.nih.gov/articles/PMC5653442/.
  28. Oliveira-Nunes, M. C.; Assad Kahn, S.; de Oliveira Barbeitas, A. L.; e Spohr, T. C.; Dubois, L. G.; Ventura Matioszek, G. M.; Querido, W.; Campanati, L.; de Brito Neto, J. M.; Lima, F. R.; Moura-Neto, V.; Carneiro, K. (2016). The availability of the embryonic TGF-β protein Nodal is dynamically regulated during glioblastoma multiforme tumorigenesis. Cancer Cell International (1 ed., vol. 16). Springer Science and Business Media LLC.
    https://cancerci.biomedcentral.com/articles/10.1186/s12935-016-0324-3.
  29. Querido, W.; Rossi, A. L.; Farina, M. (2016). The effects of strontium on bone mineral: A review on current knowledge and microanalytical approaches.. Micron (Oxford, England : 1993) (vol. 80, pp. 122-34).
    https://www.sciencedirect.com/science/article/abs/pii/S0968432815300524.
  30. Querido, W.; Farina, M.; Anselme, K. (2015). Strontium ranelate improves the interaction of osteoblastic cells with titanium substrates: Increase in cell proliferation, differentiation and matrix mineralization.. Biomatter (1 ed., vol. 5, pp. e1027847).
    https://www.tandfonline.com/doi/10.1080/21592535.2015.1027847?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%20%200pubmed.
  31. Querido, W.; Campos, A. P.; Martins Ferreira, E. H.; San Gil, R. A.; Rossi, A. M.; Farina, M. (2014). Strontium ranelate changes the composition and crystal structure of the biological bone-like apatite produced in osteoblast cell cultures.. Cell and tissue research (3 ed., vol. 357, pp. 793-801).
    https://link.springer.com/article/10.1007/s00441-014-1901-1.
  32. Rossi, A. L.; Moldovan, S.; Querido, W.; Rossi, A.; Werckmann, J.; Ersen, O.; Farina, M. (2014). Effect of strontium ranelate on bone mineral: Analysis of nanoscale compositional changes.. Micron (Oxford, England : 1993) (vol. 56, pp. 29-36).
    https://www.sciencedirect.com/science/article/abs/pii/S0968432813001510?via%3Dihub.
  33. Querido, W.; Farina, M. (2013). Strontium ranelate increases the formation of bone-like mineralized nodules in osteoblast cell cultures and leads to Sr incorporation into the intact nodules. Cell and Tissue Research (2 ed., vol. 354, pp. 573-580). Springer Science and Business Media LLC.
    https://link.springer.com/article/10.1007/s00441-013-1669-8.
  34. Querido, W.; Rossi, A. L.; Campos, A. P.; Rossi, A. M.; Farina, M. (2013). Does crystallinity of extracted bone mineral increase over storage time?. Materials Research (5 ed., vol. 16, pp. 970-974). FapUNIFESP (SciELO).
    https://www.scielo.br/j/mr/a/9SsjBkX6pb8Qp5HVg5fqcHG/?lang=en.
  35. Oliveira, J. P.; Querido, W.; Caldas, R. J.; Campos, A. P.; Abraçado, L. G.; Farina, M. (2012). Strontium is incorporated in different levels into bones and teeth of rats treated with strontium ranelate.. Calcified tissue international (3 ed., vol. 91, pp. 186-95).
    https://link.springer.com/article/10.1007/s00223-012-9625-2.
  36. Querido, W.; Farina, M.; Balduino, A. (2012). Giemsa as a fluorescent dye for mineralizing bone-like nodules in vitro.. Biomedical materials (Bristol, England) (1 ed., vol. 7, pp. 011001).
    https://iopscience.iop.org/article/10.1088/1748-6041/7/1/011001.
  37. Rossi, A. L.; Barreto, I. C.; Querido, W.; Rosa, F. P.; Rocha-Leão, M. H.; Werckmann, J.; Rossi, A. M.; Borojevic, R.; Farina, M. (2012). Ultrastructure of regenerated bone mineral surrounding hydroxyapatite-alginate composite and sintered hydroxyapatite.. Bone (1 ed., vol. 50, pp. 301-10).
    https://www.sciencedirect.com/science/article/abs/pii/S8756328211013202.
  38. Querido, W.; Abraçado, L. G.; Rossi, A. L.; Campos, A. P.; Rossi, A. M.; San Gil, R. A.; Borojevic, R.; Balduino, A.; Farina, M. (2011). Ultrastructural and mineral phase characterization of the bone-like matrix assembled in F-OST osteoblast cultures.. Calcified tissue international (5 ed., vol. 89, pp. 358-71).
    https://link.springer.com/article/10.1007/s00223-011-9526-9.
  39. Mello, A.; Hong, Z.; Rossi, A. M.; Luan, L.; Farina, M.; Querido, W.; Eon, J.; Terra, J.; Balasundaram, G.; Webster, T.; Feinerman, A.; Ellis, D. E.; Ketterson, J. B.; Ferreira, C. L. (2007). Osteoblast proliferation on hydroxyapatite thin coatings produced by right angle magnetron sputtering.. Biomedical materials (Bristol, England) (2 ed., vol. 2, pp. 67-77).
    https://iopscience.iop.org/article/10.1088/1748-6041/2/2/003.

Courses

BME 306: Introduction to Biomedical Engineering
Spring 2025, Fall 2025

BME 503: Physiology for Engineers II
Spring 2026

BME 502: Physiology for Engineers I
Fall 2026