Dr. Ueli Gubler (Visiting Professor in the Department of Chemistry and Chemical Biology) was most recently the head of Biotechnology in the Roche Research and Early Development Division. He has over 30 years’ experience in the field of gene cloning, expression of proteins and general molecular biology techniques. While working at Hoffmann-La Roche, one of Dr. Gubler’s seminal contributions to the field was the development of a simple and highly efficient method for cDNA cloning. At Roche, this technique was applied in a number of projects, resulting in original reports of the cDNA-sequences and expression of growth hormone releasing factor (GRF), preprocholecystokinin (CCK), mouse interleukin-1a and human interleukin-12. Dr. Gubler was awarded the Roche Research and Development Award for the discovery of Interleukin-12, is a co-inventor on six other issued US patents, and is an author on 79 publications.
Dr. Alvin Stern (visiting Professor in the Department of Chemistry and Chemical Biology) has 35 years of protein purification experience and is known for his pioneering work at Hoffmann-La Roche on the purification of Interleukin-2 (IL-2) using innovative chromatographic procedures. During Dr. Stern’s career, he developed unique approaches to purify difficult proteins. Some of these technologies were subsequently used in the purification of IL-1 and the IL-1 receptor. Equally novel was the purification of natural IL-12, which opened a new field of study in the differentiation of naive T cells into Th0 cells, which further develop into either Th1 or Th2 cells. He was awarded the Roche Research and Development Award for the discovery of Interleukin-12. Most recently, Dr. Stern was head of the Protein Biochemistry group at Roche and thus contributed to all Therapeutic Areas in Roche’s Discovery Research division with particular focus on purification and characterization of recombinant proteins for assay development and structural determination. He is the author or coauthor of over 65 publications and the inventor or co-inventor on four protein purification patents.
Dr. Kuo-Sen Huang (visiting Professor in the Department of Chemistry and Chemical Biology) has over 30 years of experience in protein biochemistry, assay development, high throughput screening and data management. While working at Hoffmann-La Roche, Dr. Huang purified several cell adhesion molecules such as VCAM-1, MAdCAM-1, VLA-4 and a4ß7 integrins and developed assays for screening drug antagonists. Dr. Huang and his coworkers eventually developed a potent VLA4 antagonist for Phase I and II clinical studies. Most recently, Dr. Huang was the head of Biochemical High-throughput Screening (HTS) Group in the Discovery Technology Department at Roche responsible for assay development, HTS and supporting lead discovery programs for Oncology, Metabolic Disease and Inflammation Department projects. For the past 14 years, he led a group of scientists supporting more than 80 projects and made numerous contributions. He also implemented several biophysical techniques such as. Biacore, thermal shift assay and ITC for hit validation. Prior to joining Roche, Dr. Huang was a protein biochemist at Biogen for five years, responsible for purification of therapeutic proteins. He is the author or coauthor of over 45 publications and an inventor or co-inventor of five assay and protein related patents.
Dr. William Windsor (Adjunct Professor and Visiting Scholar in the Department of Chemistry and Chemical Biology) directs the CHI Biophysical Chemistry Lab for Drug Discovery and was most recently the Director of the Biochemistry & Biophysics Department at the Merck Research Laboratory and Director of Kinase Research at Schering-Plough Research Institute (SPRI). He has over 30 years’ experience in preclinical drug discovery and is an expert in biochemical and biophysical analysis of protein-ligand / protein-protein interactions, enzymology and structure-based drug design to elucidate the mechanism of action of small molecule inhibitors. Dr. Windsor has extensive experience in methods of calorimetry (ITC, DSC), circular dichroism, absorbance and fluorescence spectroscopy and protein purification. Dr. Windsor is recognized as one of the first scientists at SPRI to develop an expertise in protein kinase biochemistry and due to his experience and leadership skills became the Chairman of a100 member cross-functional Protein Kinase Working Group which developed state of the art methods to discover and develop clinical kinase inhibitor drugs. His preclinical research focused on oncology and immunology/inflammation diseases. Noted outstanding accomplishments include the development of seven clinical drugs including: Sarasar (FPT inhibitor for Progeria and Oncology), HCV protease inhibitor Boceprevir, kinase inhibitors Dinaciclib (CDK2) and ERK inhibitor SCH 900353. Dr. Windsor was awarded the Schering-Plough Presidential Research Award for the discovery of Dinaciclib and for the X-ray Structure of FPT and is an author and co-inventor for over 50 publications and patents.
Dr. Sunil Paliwal (teaching Assistant Professor in the Department of Chemistry and Chemical Biology) leads CHI efforts in medicinal chemistry. Dr. Paliwal has 15 years experience as a medicinal chemist and project leader performing research in drug discovery for Schering-Plough Research Institute and at Merck where he was Director of Exploratory Medicinal Chemistry. Dr. Paliwal has extensive experience in developing small molecules to treat oncology, neurological, cardiovascular and inflammation diseases. He has worked on several different types of targets such as kinase, GPCR, membrane bound proteins, ion-channels, metalloprotease and extensively utilized x-ray crystallography molecular modeling to guide the design and synthesis of new organic molecules. Dr. Paliwal’s preclinical discovery includes leading teams to discover early drug hits from high-throughput screening (HTS) involving the screening of millions of compounds, optimization of hits and developing program leads through evaluations of chemical structure and biological data to develop key structural-activity-relationships (SAR). Dr. Paliwal’s research experience also include optimization of the DMPK and biological data of the leads to deliver clinical compounds with optimal DMPK/pharmacological properties. He has progressed three compounds into clinic to treat cancer and neurological diseases.
Dr. Naoko Tanaka is at the forefront of research on protein biochemistry and enzymology with over 14 years of international research experience in industrial and academic settings. At Hoffmann-La Roche, Naoko held a joint position in the Protein Biochemistry group and in the Department of Virology, where she focused on purification and characterization of viral proteins and host interacting proteins for assay development and structure determination. Prior to her experience at Roche, Naoko had worked at premier research institutions such as Memorial Sloan-Kettering Cancer Center (New York), Cancer Research UK (Herts, UK) and Weill Medical College of Cornell University (New York). During her postdoctoral training, she made a demonstrable impact on the field of protein biochemistry with her research on RNA splicing and processing. Naoko contributed to the advanced understanding of RNA splicing and its mechanism that is critical to the understanding of the cause of diseases with genetic disorders. All of her research publications, counting 13 papers as a first author, contain exquisite biochemical data and complementing genetic assays or structural information, which are the result of high quality protein/enzyme production. Naoko obtained her Ph.D. in Biochemistry/Biocatalysis from University of Amsterdam (The Netherlands).
Dr. Wei Chu has more than 20 years of experience in biomedical research and development. His original career track as a pediatric surgeon shifted into basic scientific research after earning a PhD in Molecular Biology. His research career started as a post doctoral fellow. During his post-doctoral fellowship in the Department of Molecular Genetics at Hoffmann-La Roche, Dr. Chu identified and characterized multiple novel cytokine inducible genes in human endothelial cells and identified the mechanism of tissue-specific gene regulation of adhesion molecule, E-selectin. After postdoc training, Dr. Chu stayed on with Roche and became a senior scientist. Over the years, he broaden his drug discovery knowledge base while being a member of several departments including Inflammation, Genomic Research, Biopharmaceuticals and Discovery Technologies. In the latest 14 years at Roche, his work focused on recombinant protein expression and vector engineering.
Lena Liang has extensive skills in protein biochemistry, with over 9 years of experience in field. Lena was a Senior Associate and Project Coordinator at Hoffmann-La Roche, where she was responsible for developing and executing protocols for protein production and protein characterization for the therapeutic areas. As Project Coordinator, she facilitated interdepartmental communication and collaborations with Project Managers. Prior to joining Roche, Lena worked in Dinshaw Patel’s Lab at Memorial Sloan Kettering where she was involved in elucidating epigenetic regulation using protein crystallography. Lena received her B.A. in biochemistry from New York University.
Michael Sabio has extensive experience and a highly successful track record in computational chemistry and drug design, which spans over thirty years and includes positions in Queens College, Berlex Laboratories, Inc., Sandoz / Novartis Pharmaceuticals Corporation, Lundbeck Research U.S.A., and 3D-2Drug. His extensive drug-design experience involves all stages of drug discovery amenable to computer-aided molecular modeling, e.g., in lead finding, lead optimization, potency and selectivity prediction and enhancement, scaffold identification, de novo design, depeptidization, physical property optimization, and assay-screening evaluation. Michael has expertise in most major software applications and methodologies, with programming development as needed. When he was at Novartis, his collaboration with medicinal chemistry was cited as the only instance in Novartis resulting in the development of a virtual-screening hit into a clinical candidate. Michael's impact on many drug-design projects in numerous disease areas was demonstrated by, among other achievements, becoming a co-author of 42 published articles and a co-inventor on 11 U.S. and international patents.
Yingsi Chen has over 10 years of drug discovery experience. She was a Principle Research Associate at Hoffmann-La Roche where she worked on lead optimization of drug candidates and high-throughput screening (HTS). She supported multiple discovery projects in the therapeutic areas of oncology, metabolic diseases, inflammation and virology. Her technical expertise is in development of biochemical assays of a variety of assay formats including ELISA, HTRF, FP, absorbance, and fluorescence. She developed and conducted biochemical assays to support lead optimization of over 20 discovery projects, including assay support for an advanced cyclin dependent kinase (CDK) Inhibitor project from lead series identified to clinical candidate selected. She was awarded a Roche Special Recognition Award in 2011 for her outstanding contribution to an inflammation project from assay development, HTS to lead series identification.
Frank Podlaski has experience in academia and industry including six years at Memorial Sloan Kettering and 30 years with Hoffmann La Roche. In his last ten years at Roche his role was as site expert for biosensor (BiaCore) label free assays for small molecule drug discovery. He is recognized for bringing SPR technology to the Nutley campus. Franks management of this function was pivotal in developing methods and processes to efficiently work multiple programs supporting three therapeutic areas including oncology, inflammation and metabolic diseases. His duties also included guiding contracted CROs for label free assays and due diligence evaluations of external data for prospective licensing agreements as well as responsibilities for data storage / retrieval and technology development. He produced binding data on over 20 targets for both enzyme and protein-protein interaction systems including kinases, and cytokines. Prior to the role as biophysical scientist, he functioned as biochemist and developed expertise in immunochemistry, protein purification and characterization of recombinant and natural proteins. This early work focused on cytokine research with structure-function studies of IL-2 and IL-2 receptor including development of anti-peptide antibodies and epitope mapping. Concurrently working on discovery programs for new cytokines, he was an integral part of the team that discovered IL-12. After many fruitful collaborative studies on the biology and structure of IL-12 he then transitioned to protein biochemistry department and applied his expertise to developing methods for the purification of recombinant proteins to support primary screening and other protein studies. At Sloan, his earliest work was purifying and profiling glycosphingolipids from metastasizing tumors in a lipid biochemistry laboratory then moving to a macromolecular biochemistry group that functioned as the core sequencing facility. Frank is author/coauthor on 26 publications and has one purification patent.