Mass Spectroscopy Courses

Modern Mass Spectrometry: Chemistry 666

 

Lecture and Practical Schedule

Aug 31st

Course Introduction; Review of spectroscopic techniques; basic concepts of MS; Detectors, Analyzers (magnetic, quadrupole, ion trap, TOF, FTMS), ionization Techniques (EI, CI, DCI, FD, ICP, FAB)

Sept 7th

Ionization Techniques (ESI, turbospray, nanospray, sonic-spray, photo-ionization, APCI, MALDI, SELDI)

Sept 14th

Data Acquisition, Elemental composition, accurate mass (lockspray), isotopes, molecular ion, molecular formulas, degree of unsaturation, "Rule of 13" 

Sept 21st

Basic fragmentation mechanisms, electron-ionization (EI) spectra, Interpretation of EI  spectra: Hydrocarbons, alcohols and phenols, ethers, aldehydes, ketones, acids, esters,  amines and amides. 

Sep 28th

Continuation EI spectra 

Oct 5th

Multiply charged ions, mol wt. of proteins, CAD, MS/MS, linked scans 

Oct 12th

Preliminary Exam 1

Oct 19th

Fragmentation of even-electron positive and negative ions 

Oct 26th 

Amino acids and other small molecules, peptide sequencing, proteins

Nov 2nd

Carbohydrates, nucleosides, oligonucleotides, lipids

Nov 9th 

GC-MS, derivatization, artifacts, computer identification of spectra (PBM), use of Wiley Library

Nov 16th 

Guest Lecture ?

Nov 23rd 

Thanksgiving Recess, no class

Nov 30th 

LC-MS, quantification Application of Mass Spectrometry (Environmental, Biological, Pharmacological, Forensic, Geochemical Applications), IRMS

Dec 7th

Review session.  "Why Mass Spectrometry is so difficult to understand" (each student will be given an article from literature to review; 25 bonus points). 

Dec 14th

Final Exam

Books

Mass Spectrometry
A Textbook
Gross, Jürgen H. 2004, XVIII, 518 p. 357 illus., Hardcover
ISBN: 3-540-40739-1

 

INTERPRETATION OFMASS SPECTRA4TH EDITION
Fred W. McLafferty
Cornell University

Frantisek Turecek
University of Washington

Practical Schedule

Five Saturday sessions will be held between 9 AM-12 noon.  Dates will be announced later.

Chemistry 666 | Fall 2006

 

 

 

Grading Scheme (Total 500 pts)

(All quizzes will be take-home. They will be handed out before the class and due on the following week)

Quiz #1     

(Sept 7; due Sept 14)                                    

30 pts

Quiz #2

(Sept 21; due Sept 28)

30 pts

Quiz #3

(Oct 12; due Oct 19)

30 pts

Quiz #4   

(Oct 26; due Nov 2)

30 pts

Quiz #5    

(Nov 9; due Nov 16)

30 pts

 

Prelim #1

(Oct 12, Thursday 6.30 - 9.00 PM)             

150 pts

 

Review session #1

(Dec 8, Thursday 6.15 - 9.00 PM)           

(25 bonus points)

 

Final Exam

Dec 14, 6.15-9.00 PM open book

200 pts

 

 

Total

500 pts