Lecture and Practical Schedule
Course Introduction; Review of spectroscopic techniques; basic concepts of MS; Detectors, Analyzers (magnetic, quadrupole, ion trap, TOF, FTMS), ionization Techniques (EI, CI, DCI, FD, ICP, FAB)
Ionization Techniques (ESI, turbospray, nanospray, sonic-spray, photo-ionization, APCI, MALDI, SELDI)
Data Acquisition, Elemental composition, accurate mass (lockspray), isotopes, molecular ion, molecular formulas, degree of unsaturation, "Rule of 13"
Basic fragmentation mechanisms, electron-ionization (EI) spectra, Interpretation of EI spectra: Hydrocarbons, alcohols and phenols, ethers, aldehydes, ketones, acids, esters, amines and amides.
Continuation EI spectra
Multiply charged ions, mol wt. of proteins, CAD, MS/MS, linked scans
Preliminary Exam 1
Fragmentation of even-electron positive and negative ions
Amino acids and other small molecules, peptide sequencing, proteins
Carbohydrates, nucleosides, oligonucleotides, lipids
GC-MS, derivatization, artifacts, computer identification of spectra (PBM), use of Wiley Library
Guest Lecture ?
Thanksgiving Recess, no class
LC-MS, quantification Application of Mass Spectrometry (Environmental, Biological, Pharmacological, Forensic, Geochemical Applications), IRMS
Review session. "Why Mass Spectrometry is so difficult to understand" (each student will be given an article from literature to review; 25 bonus points).
Gross, Jürgen H. 2004, XVIII, 518 p. 357 illus., Hardcover
INTERPRETATION OFMASS SPECTRA4TH EDITION
Fred W. McLafferty
University of Washington
Five Saturday sessions will be held between 9 AM-12 noon. Dates will be announced later.
Chemistry 666 | Fall 2006
Grading Scheme (Total 500 pts)
(All quizzes will be take-home. They will be handed out before the class and due on the following week)
(Sept 7; due Sept 14)
(Sept 21; due Sept 28)
(Oct 12; due Oct 19)
(Oct 26; due Nov 2)
(Nov 9; due Nov 16)
(Oct 12, Thursday 6.30 - 9.00 PM)
Review session #1
(Dec 8, Thursday 6.15 - 9.00 PM)
(25 bonus points)
Dec 14, 6.15-9.00 PM open book