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 | | Joseph S. Glavy | | Assistant Professor, Chemical Biology |  | | School: | Schaefer School of Engineering & Science | | Department: | Chemistry, Chemical Biology & Biomedical Engineering | | Program: | Chemistry & Chemical Biology
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| | Location: | 314 McLean | | Phone: | 201.216.8193 | | Fax: | 201.216.8196 | | Email: | jglavy@stevens.edu |
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CH 281:Biology and Biotechnology
Biological principles and their physical and chemical aspects are explored at the cellular and molecular level. Major emphasis is placed on cell structure, the processes of energy conversion by plant and animal cells, genetics and evolution, and applications to biotechnology. |
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CH 684:Molecular Biology Laboratory Techniques
This laboratory course introduces essential techniques in molecular biology and genetic engineering in a project format. The course includes aseptic technique and the handling of microbes; isolation and purification of nucleic acids; construction, selection and analysis of recombinant DNA molecules; restriction mapping; immobilization and hybridization of nucleic acids; and labeling methods of nucleic acid probes. |
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CH 687:Molecular Genetics
This course is a modern approach to the study of heredity through molecular biology. Primary emphasis is on nucleic acids, the molecular biology of gene expression, molecular recognition and signal transduction, and bacterial and viral molecular biology. The course will also discuss recombinant DNA technology and its impact on science and medicine. |
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CH 700:Seminar in Chemistry
Lectures by department faculty, guest speakers, and doctoral students on recent research. |
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CH 496:Chemistry Project I
Participation in a small group project, under the guidance of a faculty member, whose prior approval is required. Experimentation, application of chemical knowledge and developmental research leading to the implementation of a working chemical process. Individual or group written report required. |
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CH 497:Chemistry Project II
Participation in a small group project, under the guidance of a faculty member, whose prior approval is required. Experimentation, application of chemical knowledge and developmental research leading to the implementation of a working chemical process. Individual or group written report required.
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CH 498:Chemical Research I
Individual research project under the guidance of a chemistry faculty member, whose prior approval is required. A written report in acceptable journal format and an oral presentation are required at the end of the project.
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CH 499:Chemical Research II
Individual research project under the guidance of a chemistry faculty member, whose prior approval is required. A written report in acceptable journal format and an oral presentation are required at the end of the project.
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| | Research & Education | |
| | | | Research | |
The Nuclear Pore Complex and Mitosis The long-range goal of the Glavy Lab is to outline the molecular mechanisms of the mitotic breakdown of the nuclear pore complex (NPC) with a main focus on the effects of cell cycle dependent modifications on the regulation of nucleoporins (starting with the Nup107-160 subcomplex) and hence the NPC. The NPC is the principal passageway for nucleocytoplasmic macromolecular traffic. It is composed of about 30 proteins, termed nucleoporins (Nups) designated by their molecular weights in kilodaltons. These are organized into a pseudo-symmetric structure with a two-fold plane quasi-parallel to the nuclear envelope and an eight-fold axis of symmetry across the nucleo-cytoplasmic axis. Most of the Nups are part of a symmetric core structure and therefore occur in at least 16 copies per NPC (e.g. Nup107, Nup133). Others are present only on either the cytoplasmic or the nucleoplasmic side and are present in eight copies per NPC (e.g. Nup358, Nup214, Nup153 also designated FG Nups because of their regional FG (Phe-Gly) sequence repeats). FG Nups are targets of at least nine chromosomal rearrangements found in leukemia, primarily in acute myelogenous leukemia (AML) and myelodysplastic syndrome (MDS). These chromosomal translocations further effect other related Nups, such as Nup107 and Nup133, demonstrating their interdependence which is critical during cell division.
The NPC is disassembled into subcomplexes of Nups during open mitosis in higher eukaryotic cells. Mitotic disassembly is thought to be triggered by phosphorylation. The best characterized subcomplex is the vertebrate Nup107-160 and the homologous Nup84 subcomplex in Saccharomyces cerevisiae. The Nup107-160 subcomplex has 9 members (Nup160, Nup133, Nup107, Nup96, Nup75, Nup43, Nup37, Seh1 and Sec 13. In the “protocoatomer” hypothesis, this nonameric complex has been proposed to stabilize the sharp bend between the inner and outer nuclear envelope membrane.  Visit the Glavy Lab 
| | Education | | Postdoctoral Training under Dr. Günter Blobel, Laboratory of Cell Biology, Rockefeller University, NY, NY. 1999: Ph.D. Department of Molecular Pharmacology, Albert Einstein College of Medicine, Yeshiva University, Bronx, NY.
1993: Master of Science. Roswell Park Cancer Institute, Buffalo, NY.
1989: Bachelor of Science. Major: Biology. State University of New York at Buffalo, NY. |
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| | Selected Publications | |
| Journals
"Recent Publications.....".
Blethrow, J.D., Glavy, J.S. Morgan, D.O., and Shokat K.M.. (2008). "Covalent capture of kinase-specific phosphopeptides reveals Cdk1-cyclin B substrates. ", PNAS, 105 1442-1447.
Glavy, J. S., Krutchinsky, A., Cristea, I.M., Berke, I.C., Boehmer, T., Blobel, G. and Chait, B.T. (2007). "Cell-Cycle Dependent Phosphorylation of the Nuclear Pore Nup107-160 Subcomplex", PNAS, 104 3811-3816.
Book Chapters
Glavy, J.S.. (2009). "Nuclear Pore Complex", The Liver: Biology and Pathobiology. , John Wiley & Sons Press. in press.
Blethrow, J.D. and Glavy, J.S.. (2009). "Purification of Cdk1-Cyclin B Kinase Targeted Phosphopeptides from the Nuclear Envelope", Methods in Molecular Biology: Nuclear Envelope: Methods and Protocols, The Humana Press Inc.. in press.
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