Nonlinear spectroscopies provide powerful tools for probing biological systems. This talk will discuss applications of different nonlinear spectroscopies for imaging and condensed phase studies. For biological imaging, Coherent anti-Stokes Raman scattering (CARS) offers the advantage of providing endogenous contrast based on the inherent vibrations of different chemical species. We present a time-domain Fourier transform-based method that produces spectrally resolved CARS images over the considerable bandwidth of a broadband laser source, and discuss recent results in applying this method. For studying energy transfer and condensed phase dynamics, two-dimensional electronic spectroscopy (2DES) has recently emerged as a powerful
technique. Despite the rich chemical information available from 2DES, the relative difficulty of implementing the experiment has limited the degree to which the method has been utilized. We present a simple experimental implementation of 2DES and discuss recent and future applications of the method.
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