Organic Conducting Polymer/CMOS Field Effect Transistor Hybrid for Biodectection

Friday, February 7, 2014 ( 2:00 pm to 3:00 pm )

Location: McLean 218A, Stevens Institute of Technology

Nancy Webb,

Dr. Kalle Levon
Polytechnic Institute of NYU
Department of Chemical and Biomolecular Engineering
Semiconductor-based diagnostics have revolutionized genome sequencing as ion-sensitive field effect system has been applied for monitoring the pH changes during the polymerization of DNA. Similar charge-dependent changes can be monitored during protein-protein binding as in antibody-antigen reaction. We have designed and had manufactured autonomic floating gate (FG) field-effect transistor (FET) array and use it for monitoring protein bindings and enzymatic reactions. The advantage is that with a floating, one doesn't have to “scan” for threshold voltage as the floating is between control gate and the source/drain pair only causing shifts in the already controlled threshold voltage. The additional unique feature is our hybrid system in which we apply organic semiconducting materials on top of the floating gate optimizing the amplification from the eventual field effect. Additionally, the development nanostructured architecture of the organic semiconductor optimized the ligand density and immobilization. In our surfaces, we have applied covalent thiol- or glutaraldehyde-based conjugations with the benzoid amine or quinoid imine groups of polyaniline. We have first used the FET array in both IS (with reference electrode) and FGFET methods to follow pH changes and bindings of cationic and anionic polyelectrolytes. Further on, we followed the change in the threshold voltage protein binding on the surface, the selective protein binding on the surface protein and finally also the effect of enzymatic substrate reacting with the conjugated enzyme.
Kalle Levon is an expert in the field of organic electronics, with particular interest in medical diagnostics. Using organic electronics as early-stage cancer markers, Levon focuses his efforts on assisting medical doctors with point-of-care diagnostics. Levon joined the faculty of Polytechnic Institute of NYU in 1989 as an assistant professor of polymer chemistry. He became an associate professor in 1993 and department head in 1995. He successfully pioneered the department’s transition from chemistry to biological sciences and engineering and introduced degree programs in biomedical engineering, biomedical sciences, and bioinformatics. During this time, he also became head of the Polymer Research Institute (1996-2003). As associate provost of research and intellectual property (2003-2006), Levon focused primarily on the Institute’s patent portfolio. While in this position, he founded the Brooklyn Enterprise for Science and Technology (BEST). He developed courses in biosensors, tissue engineering, sequence analysis, and protein structure/property relationship. His $5 million in research funding during a 10-year period led to novel discoveries in bacteria, virus, protein and DNA detection.
Levon earned his Bachelor of Science and Master of Science in Organic Chemistry from Helsinki University in 1976 and 1981, respectively, and a PhD in Agriculture from Tokyo University in 1986. He is a recipient of the Wechsler Award for Excellence, the Search for Electroactive Materials Award, the Othmer Institute Award and the FiDIPro Finnish Distinguished Professor Award.