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Laboratory for Multiscale Imaging

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FIB-SEM Cryo Workshop



Instruments

Major Equipments

Supporting Equipment

  • Gatan cryo-plunger
  • Gatan Dry Pumping station
  • Olympus optical microscope
  • thermo evaporator
  • sputter coater
  • Leica Ultracut cryo/microtome

User Charge

See below table.

FEI CM20 field-emission S/TEM

FEI CM20 FE S/TEM with a Schottky field emitter and a TWIN pole pieces objective lens : 0.282 nm point resolution, 0.144 nm line resolution, and 0.180 nm information limit at 200 kV (TEM mode), and 1.0 nm BF/DF STEM resolution.
Aperture holderpos.1pos.2pos.3pos.4
Condenser 12 mm70 um50 um30 um
Condenser 2150 um100 um50 um30 um
Objective100 um40 um20 um10 um
Select-area800 um200 um40 um10 um

Energy Spread : <= 0.8 eV (Extr. Voltage < 3 kV) and <= 1 eV (Extr. Voltage > 3 kV)
With  Gatan 792 Multi-scan CCD (1kx1k) camera
Gatan Enfina PEELS system
Gatan On-axis BF/DF STEM detector
Gatan Digi-Scan digital STEM data acquisition system
FEI Emispec digital STEM data acquisition system
Gatan cryo-shield for cryo S/TEM
off-axis TV rate camera
various room temperature and low temperature TEM holders.

Regarding to basic information of becoming a self-user, hourly rate, training and etc., please click here to download the LMSI TEM Users Guide.  Please contact Dr. Tsengming (Alex) Chou at 201-216-8310 if you have any questions.

Zeiss 982 field-emission SEM

coming soon......

Zeiss 982 FE-SEM with Oxford ISIS EDS system SEM resolution : 1 nm @ 30 kV ;4 nm @  1 kV Detectors: Convectional E-T detector, in-lens detector, 4-diodes solid-state BSE detector   Oxford ISIS EDS system with 135 eV resolution and it is capable of collecting spectrum from multiples points, lines across the interface, and elemental mapping.

It also equips a home-made e-beamlithography system with 100 nm or so resolution.

Regarding to basic information of becoming a self-user, hourly rate, training and etc., please click here to download the LMSI SEM Users Guide.  Please contact Dr. Tsengming (Alex) Chou at 201-216-8310 or Dr. Berton Greenbergat 201-216-5258 if you have any questions.

Zeiss Auriga Small Dual-Beam FIB-SEM

Nanotechnology WorkstationAuriga : combine both FE-SEM and HR-FIB, sample can be manipulated in nano-scale.

 

High Resolution FESEM

a GEMINI field-emission electron column that provides resolution of 1.0 nm @ 15 kV and 1.9 nm @ 1 kV. 

 

This FE-SEM can:

magnification: 12x to 1000 kx

probe current: 4pA to 20 nA

HV: 0.1 to 30 kV

with in-lens SE detector, Everhart-Thornley type SE chamber detector, in-lens EsB (energy selective backscattering) detector, and a STEM BF/DF detector for the thin specimen.

N2 gas charge compensation device is also available.

 

High Resolution FIB

Resolution down to 2.5 nm @ 30 kV via this Cobra ion column

source: liquid metal gallium ion source

magnification: 300x to 500kx

probe current: 1 pA to 50 nA

HV: 1.0 to 30 kV

with Pt GIS (gas injection system) to provide both electron and ion beam induced deposition.

 

Introduction to 3D Reconstruction

Image stack of serial sectioned images at nano-scale can be produced on this system.  Using Avizo 3D reconstruction and visualization platform, studies of 3D nano-structure can be achived. 

 

High Resolution STEM Imaging

Using the retractable BF/DF STEM detector, sample can be imaged in STEM (scanning transmission electron microscopy) mode via electrons have up to 30 kV energy.  It provides structure information on a thin specimen.

 

TEM Sample Preparation

Automatic TEM sample preparation can be done using in-situ lift out method via Kleindiek nano-manipulator (model: MM3A).

 

Advanced Analytical Platform

A 80 mm2 Oxford SDD (silicon drift detector) EDS system provides a high through-put and high resolution microanalysis capability.

 

Electron and Ion Beam Lithography

Via Nabity's NPGS system, lithography can be done via either electron or ion beam.

 

Cryo-capability

A Leica VCT cryo-transfer system is coming soon to provide a cryo-EM capability on this system.


NanoInk DPN / Aglient NanoR AFM

For AFM mode only using Agilent SPM Cockpit program:  a simple instruction can be download here.  For an official overview, click here to download the document.  A guided page of using NanoR in contact mode, click here to download the document.  A guided page of using NanoR in non-/close-contact mode, click here to download the document.

For DPN users, click here to download the Quick user guide prepared by NanoInk.  For a complete user manual, click here to download.  InkCAD program can also image the sample surface similar to SPM Cockpit program, but with a limit capability.

Nikon E-1000 + C1 LSCM

Nikon E-1000 up-right florescent microscope with automatic stage control.  

A high sensitive monochromatic CCD camera for florescent imaging

Nikon C-1 LSCM with 3 lasers

Regarding to basic information of becoming a self-user, hourly rate, training and etc., please click here to download the LMSI Nikon/Confocal Users Guide.  Regarding to the basic laser safety information when using the Confocal system, please visit Nikon Microscopy University web-site and search for the laser safety information.  Or, download the printable version of the Nikon document by clicking here.  Please contact Dr. Tsengming (Alex) Chou at 201-216-8310 if you have any questions.

User Fee


Instrument

Stevens users

with assistance

outside users

with assistance

TEM

$50/hr

$ 50+35/hr

$150/hr

$ 150+100/hr

SEM

$50/hr

$ 50+35/hr

$150/hr

$ 150+100/hr

AFM

$10/hr

$ 10+35/hr

$50/hr

n/a

Nikon*

$10/hr

$ 10+35/hr

$50/hr

n/a

TEM training: 2 hrs of lectures and 2 to 4 hrs of hands on practice.  $200 for internal Stevens users and $2,000 for external users

SEM training: 2 hrs of video training, 2 to 4 hrs of hands on practice.  $200 for internal Stevens users and $2,000 for external users

AFM and Nikon training will be arranged separately. Please consult with LMSI staff.

*Nikon E-1000 up-right light microscope with Epi-florescent, phase contrast, and LSCM (laser-scanning confocal microscopy).

 
More information

Dr. Matthew Libera
Burchard Building
Room B-010
Phone: 201.216.5259
Fax: 201.216.8306
Email: mlibera@stevens.edu

Dr. Tsengming (Alex) Chou
prof
Burchard
Room B-004
Phone: 201-216-8310
Fax: 201-216-8306
Email: Tseng-Ming.Chou@stevens.edu

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